3LJ8
Crystal Structure of MKP-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-05-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.926, 91.926, 56.605 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.680 - 2.700 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zzw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.580 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.032 | 0.228 |
| Number of reflections | 7730 | |
| <I/σ(I)> | 14.7 | 3.4 |
| Completeness [%] | 98.3 | 98.5 |
| Redundancy | 5.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% ethanol, 1.3M NaCl, 0.2M MgSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
