3LIZ
crystal structure of bla g 2 complexed with Fab 4C3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.213, 105.285, 109.154 |
| Unit cell angles | 90.00, 132.58, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.800 |
| R-factor | 0.17805 |
| Rwork | 0.177 |
| R-free | 0.20226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Bla g 2 and 7C11 antibody |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 118390 | |
| <I/σ(I)> | 17.9 | 2.4 |
| Completeness [%] | 99.3 | 94.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 20% PEG8000, 8% Ethylene glycol, 5mM DTT and 0.2mM CdCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
