3LHA

Crystal structure of mouse VPS26B(R240S/G241A/E242S) in spacegroup P41 21 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-27
Wavelength(s)	0.95364
Spacegroup name	P 41 21 2
Unit cell lengths	118.140, 118.140, 193.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.853 - 2.800
R-factor	0.2539
Rwork	0.252
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r51
RMSD bond length	0.010
RMSD bond angle	1.314
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.112	0.609
Number of reflections	64306
<I/σ(I)>	19.7	3.6
Completeness [%]	100.0	100
Redundancy	10.6	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	2M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K