3LGW
H198P/T167V double mutant of DegS-deltaPDZ protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5416 |
| Spacegroup name | H 3 |
| Unit cell lengths | 70.664, 70.664, 121.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.665 - 2.500 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.844 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.034 | 0.029 | 0.138 |
| Number of reflections | 7346 | ||
| <I/σ(I)> | 25.9 | ||
| Completeness [%] | 94.3 | 99.4 | 89.4 |
| Redundancy | 4.2 | 4.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 50 mM Sodium Cacodylate, 50-125 mM Sodium Citrate, 10-20 % isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
