3LGH
Crystal structure of NikR from Helicobacter pylori with variable Ni site coordination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2009-02-08 |
| Detector | RAYONIX MX-325 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.479, 105.056, 48.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.340 - 2.370 |
| R-factor | 0.26294 |
| Rwork | 0.260 |
| R-free | 0.31816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cad |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.434 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.370 |
| Number of reflections | 16660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294.5 | 50mM MES pH5.6, 200 mM potassium chloride, 10 mM magnesium sulfate, 5% PEG8000, vapor diffusion, sitting drop, temperature 294.5K |
