3LG8
Crystal structure of the C-terminal part of subunit E (E101-206) from Methanocaldococcus jannaschii of A1AO ATP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.658, 73.658, 149.643 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.270 - 4.100 |
| R-factor | 0.356 |
| Rwork | 0.353 |
| R-free | 0.38100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dm9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.847 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.170 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.068 | 0.076 |
| Number of reflections | 2125 | |
| <I/σ(I)> | 27.35 | 26.35 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 10.8 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M Cesium chloride, 0.1M MES monohydrate buffer (pH 6.5), 30% v/v Jeffamine M-600, 1mM TCEP, 0.1mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
