3LFZ

Crystal Structure of Protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homolog of g-AMPK.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	H 3 2
Unit cell lengths	109.144, 109.144, 142.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.810 - 2.200
R-factor	0.25324
Rwork	0.251
R-free	0.28525
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kpd
RMSD bond length	0.007
RMSD bond angle	1.230
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	78.810
High resolution limit [Å]	2.200
Number of reflections	15961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ligand soaks, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K