3LFO
Crystal structure of T. celer L30e E90A/R92A variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.913, 53.097, 33.466 |
| Unit cell angles | 90.00, 108.57, 90.00 |
Refinement procedure
| Resolution | 31.720 - 1.800 |
| R-factor | 0.16701 |
| Rwork | 0.165 |
| R-free | 0.20385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.720 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | 0.244 |
| Number of reflections | 7279 | |
| <I/σ(I)> | 14.8 | 3.7 |
| Completeness [%] | 98.1 | 96.3 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 18% PEG 3350, 0.1M sodium citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
