3LF3
Crystal Structure of Fast Fluorescent Timer Fast-FT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.838, 42.517, 61.766 |
| Unit cell angles | 90.00, 112.07, 90.00 |
Refinement procedure
| Resolution | 29.762 - 1.150 |
| R-factor | 0.15 |
| Rwork | 0.149 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.538 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 2.480 | 1.150 |
| Rmerge | 0.048 | 0.034 | 0.457 |
| Number of reflections | 83298 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.7 | 99.7 | 97.3 |
| Redundancy | 3.6 | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M MgCl2, 0.1M Tris, 30% w/v PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
