3LES
2F5 Epitope scaffold ES2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.257, 49.987, 57.567 |
| Unit cell angles | 78.03, 89.83, 87.57 |
Refinement procedure
| Resolution | 48.850 - 2.770 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ku2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.751 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 9968 | |
| <I/σ(I)> | 15 | 1.49 |
| Completeness [%] | 95.9 | 89.4 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 23% PEG 4000, 0.12 M (NH4)2SO4, 0.02 M ATP, VAPOR DIFFUSION, TEMPERATURE 293K |
