3LDA
Yeast Rad51 H352Y Filament Interface Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320357 |
| Spacegroup name | P 61 |
| Unit cell lengths | 78.905, 78.905, 130.333 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.200 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1szp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.663 |
| Number of reflections | 14791 | |
| <I/σ(I)> | 18.03 | 2.03 |
| Completeness [%] | 92.8 | 98.2 |
| Redundancy | 9.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 35% (v/v) Pentaerythritol propoxylate (5/4 PO/OH), 300mM KCl, 50mM HEPES, 20mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
