3LCK

THE KINASE DOMAIN OF HUMAN LYMPHOCYTE KINASE (LCK), ACTIVATED FORM (AUTO-PHOSPHORYLATED ON TYR394)

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-07-21
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	42.040, 73.620, 91.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.178
Rwork	0.178
R-free	0.19900
Structure solution method	MIR, MOLECULAR REPLACEMENT, MAD
Starting model (for MR)	1irk
RMSD bond length	0.005
RMSD bond angle	22.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MADSYS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.028 *	0.047 *
Number of reflections	31599
<I/σ(I)>	37	21.8
Completeness [%]	99.0	96.1
Redundancy	8.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.51 (mg/ml)
2	1	drop	ATP	0.080 (mM)
3	1	drop	substrate peptide	0.080 (mM)
4	1	drop	ammonium sulfate	0.6 (M)
5	1	drop	Bis-Tris	0.05 (M)
6	1	reservoir	ammonium sulfate	1.6 (M)
7	1	reservoir	bis-Tris	0.1 (M)