3LCA
Structure of Tom71 complexed with Hsp70 Ssa1 C terminal tail indicating conformational plasticity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.148, 97.971, 129.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.190 |
| R-factor | 0.22942 |
| Rwork | 0.227 |
| R-free | 0.27112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fp4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.095 | 0.430 |
| Number of reflections | 33761 | |
| <I/σ(I)> | 30.8 | 2.5 |
| Completeness [%] | 98.6 | 89.5 |
| Redundancy | 5.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% Ethylene Glycol, 20% PEG 6000, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
