3LBG
Urate oxidase complexed with 8-thio xanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.948 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.637, 95.471, 104.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.24100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l8w |
| RMSD bond length | 0.035 |
| RMSD bond angle | 0.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 1.650 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.880 |
| Number of reflections | 60541 | |
| <I/σ(I)> | 29.4 | 5.1 |
| Completeness [%] | 94.5 | 96 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | 0.05M TRIS BUFFER, PH 8, 10 % PEG 8000, temperature 291K |
