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3LAP

The Structure of the Intermediate Complex of the Arginine Repressor from Mycobacterium tuberculosis Bound to its DNA Operator and L-canavanine.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyCCD
Collection date2009-04-16
DetectorMARMOSAIC 325 mm CCD
Wavelength(s)0.97946
Spacegroup nameC 1 2 1
Unit cell lengths184.750, 106.532, 117.752
Unit cell angles90.00, 121.40, 90.00
Refinement procedure
Resolution39.004 - 2.150
R-factor0.1855
Rwork0.184
R-free0.20490
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle1.366
Data reduction softwareMOSFLM
Data scaling softwareHKL-2000
Refinement softwarePHENIX ((phenix.refine: 1.5_2))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.230
High resolution limit [Å]2.1502.150
Rmerge0.0670.433
Number of reflections96776
<I/σ(I)>11.51.9
Completeness [%]91.895.1
Redundancy2.42.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.52951M ammonium sulfate, 100 mM bis-tris buffer, 1% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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