3LA6
Octameric kinase domain of the E. coli tyrosine kinase Wzc with bound ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 245.165, 137.782, 158.958 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 49.740 - 3.200 |
| R-factor | 0.1863 |
| Rwork | 0.186 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ved |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (2.1.1) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 158.742 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.093 | 0.526 |
| Number of reflections | 83937 | |
| <I/σ(I)> | 11.1 | 1.2 |
| Completeness [%] | 96.5 | 97.9 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 290 | 14% PEG 4000, 0.1M Hepes, 0.1M NaCl, 0.15M CaCl2, 20% NaI 1.0M, pH 7.5, VAPOR DIFFUSION, temperature 290K |
