3L9Z

Crystal Structure of UreE from Helicobacter pylori (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 64 2 2
Unit cell lengths	131.011, 131.011, 51.267
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.880 - 2.080
R-factor	0.22277
Rwork	0.221
R-free	0.25488
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.309
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.880	50.000	2.150
High resolution limit [Å]	2.080	4.480	2.080
Rmerge	0.050	0.025	0.496
Number of reflections	16040
<I/σ(I)>	18.7
Completeness [%]	99.8	98.5	99.5
Redundancy	15.7	19.1	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	17% PEG 8000, 0.1M Sodium Citrate pH 5.0, vapor diffusion, sitting drop, temperature 295K