3L9W
KefC C-terminal domain in complex with KefF and GSH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.764, 85.852, 189.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.460 - 1.750 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.686 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.077 | 0.075 | 0.249 |
| Number of reflections | 96197 | ||
| <I/σ(I)> | 17.7 | ||
| Completeness [%] | 90.4 | 92.7 | 50 |
| Redundancy | 3.8 | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 9% PEG 3350, 100mM ammonium sulfate, 100mM Bis-Tris buffer, 50mM magnesium formate, 1mM NAD+, pH 5.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
