3L9I
Myosin VI nucleotide-free (mdinsert2) L310G mutant crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.540, 104.400, 90.330 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.200 |
| R-factor | 0.18711 |
| Rwork | 0.185 |
| R-free | 0.23471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bkh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 64176 | |
| <I/σ(I)> | 14.25 | 3.77 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 4% PEG 8000, 50mM Glycine pH9, 3% iso-propanol, 3% tert-butanol, 1mM TCEP , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
