3L9A
Structure of the C-terminal domain from a Streptococcus mutans hypothetical
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915, 0.97942 |
| Spacegroup name | I 4 |
| Unit cell lengths | 64.080, 64.080, 39.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.290 - 1.300 |
| R-factor | 0.13462 |
| Rwork | 0.134 |
| R-free | 0.15490 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.290 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.038 | 0.113 |
| Number of reflections | 20493 | |
| <I/σ(I)> | 59.333 | 10.5 |
| Completeness [%] | 90.9 | 49.9 |
| Redundancy | 6.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Hepes ph7.5, 0.2M Ammonium Sulfate, 25% PEG3350, 1/10 Papain. Cryoprotected with crystallization solution plus 8% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
