3L8U

Crystal structure of SMU.1707c, a putative rRNA methyltransferase from streptococcus mutans UA159

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-22
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	82.277, 82.277, 70.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.600 - 2.000
R-factor	0.20763
Rwork	0.206
R-free	0.23896
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e5y
RMSD bond length	0.020
RMSD bond angle	1.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	31772
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.5M (NH4)2SO4, 0.1M Bis-tris pH7.0, 0.7% beta-OG , VAPOR DIFFUSION, SITTING DROP, temperature 293K