3L8Q
Structure analysis of the type II cohesin dyad from the adaptor ScaA scaffoldin of Acetivibrio cellulolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.930 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.225, 73.912, 90.793 |
| Unit cell angles | 100.25, 94.26, 112.15 |
Refinement procedure
| Resolution | 29.630 - 1.570 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.22554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.077 | 0.446 |
| Total number of observations | 707451 | |
| Number of reflections | 192139 | |
| <I/σ(I)> | 23.9 | 2.1 |
| Completeness [%] | 97.0 | 95.4 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.15M citric acid pH 3.5, 16% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
