3L81

Crystal structure of adaptor protein complex 4 (AP-4) mu4 subunit C-terminal domain, in complex with a sorting peptide from the amyloid precursor protein (APP)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794, 0.9796, 0.9719
Spacegroup name	P 1 21 1
Unit cell lengths	46.672, 56.910, 60.664
Unit cell angles	90.00, 106.53, 90.00

Refinement procedure

Resolution	41.670 - 1.600
R-factor	0.209
Rwork	0.207
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.731
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Rmerge	0.097
Number of reflections	39268
<I/σ(I)>	13.8
Completeness [%]	97.3
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	10% PEG 3350, 10mM magnesium chloride, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	10% PEG 3350, 10mM magnesium chloride, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K