3L7H
Insights into dynein assembly from a dynein intermediate chain light chain Roadblock structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 133.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.218, 58.693, 65.095 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 63.720 - 1.950 |
| R-factor | 0.19938 |
| Rwork | 0.193 |
| R-free | 0.25417 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hz5 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.931 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.720 | 2.001 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.139 | 0.092 |
| Number of reflections | 27041 | |
| <I/σ(I)> | 2.5 | 5.5 |
| Completeness [%] | 98.9 | 94.7 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277.15 | 20% iso-propanol, 20% PEG 4000, 100 mM sodium citrate., pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
