3L7G
Crystal structure of organophosphate anhydrolase/prolidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 124.354, 143.934, 219.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.730 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l24 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.444 |
| Number of reflections | 54263 | |
| <I/σ(I)> | 9.2 | 2.2 |
| Completeness [%] | 95.8 | 97.1 |
| Redundancy | 4.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 1mM Mipafox, 16% PEG 4000, 20 mM MnCl2, 1mM beta-mercaptoethanol, 270 mM ammonium acetate and 60 mM sodium acetate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
