3L7F

Structure of IL-13 antibody H2L6, A humanized variant OF C836

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	CCD
Collection date	2008-01-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	69.640, 228.160, 234.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.20667
Rwork	0.205
R-free	0.24264
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l5x
RMSD bond length	0.010
RMSD bond angle	1.352
Data reduction software	d*TREK (9.6L)
Data scaling software	d*TREK (9.6L)
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.600	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.087	0.420
Number of reflections	56212
<I/σ(I)>	9.6	2.3
Completeness [%]	97.1	92.8
Redundancy	7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES PH 6.5, 5% PEG 4K, 38% MPD; CRYO CONDITIONS: 0.1 M MES PH 6.5, 5% PEG 4K, 40% MPD, 15% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K