3L7C
Crystal Structure of Glycogen Phosphorylase DK4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 289 |
Detector technology | CCD |
Collection date | 2009-07-29 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 129.096, 129.096, 116.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.930 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.20700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.090 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.290 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 74225 | |
<I/σ(I)> | 20.8 | 4.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10MM BES BUFFER, 3MM DDT, pH 6.7, small tubes, temperature 289K |