3L7B
Crystal Structure of Glycogen Phosphorylase DK3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.113, 129.113, 116.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.710 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.168 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.000 | 99.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.071 | 0.046 | 0.487 |
| Number of reflections | 66691 | ||
| <I/σ(I)> | 16.1 | ||
| Completeness [%] | 99.2 | 98.5 | 98.8 |
| Redundancy | 8 | 7.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10MM BES BUFFER, 3MM DDT, pH 6.7, small tubes, temperature 289K |
