3L72

Chicken cytochrome BC1 complex with kresoxim-I-dimethyl bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	172.614, 181.548, 241.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.930 - 3.060
R-factor	0.264
Rwork	0.264
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT USING NATIVE STRUCTURE SOLVED BY THE SAME AUTHOR
Starting model (for MR)	1bcc
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	141091
<I/σ(I)>	8.48	0.656
Completeness [%]	93.2	85.7
Redundancy	3.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	20MM KMES PH 6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K