3L6T
Crystal Structure of an N-terminal Mutant of the Plasmid pCU1 TraI Relaxase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.385, 58.656, 86.532 |
Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
Resolution | 31.374 - 1.930 |
R-factor | 0.1694 |
Rwork | 0.168 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omh Plasmid R388 TrwC Relaxase Domain |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.2) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 36385 | |
<I/σ(I)> | 31.589 | 3.03 |
Completeness [%] | 95.5 | 76 |
Redundancy | 7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 250mM triNa citrate, 22% PEG 3350, 5mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |