3L5W
Crystal structure of the complex between IL-13 and C836 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-31 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.620, 65.560, 118.740 |
| Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Q9Q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.424 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.415 |
| Number of reflections | 76032 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 5.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.1 M HEPES PH 7.5, 20% PEG 3350, 0.2 M SODIUM TARTRATE; CRYO CONDITIONS: MOTHER LIQUOR + 18% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
