3L4C
Structural basis of membrane-targeting by Dock180
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.995, 63.529, 63.421 |
| Unit cell angles | 90.00, 109.28, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.370 |
| Rwork | 0.216 |
| R-free | 0.26900 |
| Structure solution method | MR |
| Starting model (for MR) | 2bwq |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (& CNS) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 14369 | |
| <I/σ(I)> | 22.6 | 6.9 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch method under oil | 8.8 | 293 | 30% (w/v) polyethylene glycol 10K, 0.2 M Na-acetate, 0.1 M Tris-HCl pH 8.8, microbatch method under oil, temperature 293K |
