3L4A
Crystal Structure of an Anopheles gambiae Odorant-binding Protein 22a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.194, 37.729, 43.703 |
| Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
| Resolution | 25.465 - 1.500 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l47 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.846 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.036 | 0.026 | 0.181 |
| Number of reflections | 17623 | ||
| <I/σ(I)> | 38.2 | ||
| Completeness [%] | 94.0 | 99.6 | 82.8 |
| Redundancy | 4.2 | 6.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium citrate tribasic, MPD, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
