3L40
Crystal Structure of S. pombe Brc1 BRCT5-BRCT6 domains
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.111, 0.9796, 0.9797, 1.0087 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.472, 83.425, 87.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.550 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.22500 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.785 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 61324 | |
<I/σ(I)> | 38.6 | 2.2 |
Completeness [%] | 94.8 | 67 |
Redundancy | 6.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 295 | 20-22% w/v PEG 3350, 100 mM NaCl, 100 mM K2HPO4/KH2PO4 pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |