3L3U
Crystal structure of the HIV-1 integrase core domain to 1.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900020 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.550, 62.434, 81.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.010 - 1.400 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exq |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.079 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.015 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.112 | 0.439 |
| Number of reflections | 60131 | |
| <I/σ(I)> | 15.2 | 6.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.5 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.8M Ammonium sulfate, 0.15M sodium citrate, 5mM cadmium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
