3L3F
Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9789, 0.9792, 0.9640 |
Spacegroup name | P 61 |
Unit cell lengths | 103.115, 103.115, 72.117 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.426 - 1.900 |
R-factor | 0.19594 |
Rwork | 0.195 |
R-free | 0.21877 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.750 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.062 | 0.256 |
Number of reflections | 34591 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris-HCl (pH 7.5), 28% PEG 3350, 0.1M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |