3L3B
Crystal structure of isoprenoid biosynthesis protein with amidotransferase-like domain from Ehrlichia Chaffeensis at 1.90A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.210, 69.220, 242.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.560 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb deposition 1vhq modified with CCP4 program CHAINSAW |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.528 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.465 |
| Number of reflections | 32534 | |
| <I/σ(I)> | 20.59 | 4.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | EBS JCSG+ SCREEN G10: 150mM Postassium bromice, 30% PEG 2000 MME; EHCHA.01061.A AT 20MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
