3L2U

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and GS9137 (Elvitegravir)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2009-10-13
Detector	ADSC
Wavelength(s)	0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	157.100, 157.100, 124.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.440 - 3.150
R-factor	0.19416
Rwork	0.193
R-free	0.21836
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dlr
RMSD bond length	0.011
RMSD bond angle	1.551
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.102	3.320
High resolution limit [Å]	3.150	3.150
Number of reflections	27284
Completeness [%]	99.3	100
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.35M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50mM Mes-NaOH, pH 6.5, vapor diffusion, hanging drop, temperature 291K