3L2U
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and GS9137 (Elvitegravir)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2009-10-13 |
Detector | ADSC |
Wavelength(s) | 0.9796 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 157.100, 157.100, 124.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.440 - 3.150 |
R-factor | 0.19416 |
Rwork | 0.193 |
R-free | 0.21836 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dlr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.551 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.102 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 27284 | |
Completeness [%] | 99.3 | 100 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50mM Mes-NaOH, pH 6.5, vapor diffusion, hanging drop, temperature 291K |