3L29

Crystal Structure of Zaire Ebola VP35 interferon inhibitory domain K319A/R322A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-12
Detector	NOIR-1
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	51.430, 66.070, 72.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.320 - 1.700
R-factor	0.19349
Rwork	0.191
R-free	0.23155
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fke
RMSD bond length	0.017
RMSD bond angle	1.490
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.320	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.089	0.585
Number of reflections	27195
<I/σ(I)>	9.4	2.8
Completeness [%]	97.5	88.3
Redundancy	6.34	4.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.75	293	0.1M BIS-TRIS, 0.2M AMMONIUM SULFATE, 28% PEG3350, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K