3L1Y
Crystal structure of human UBC4 E2 conjugating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.230, 59.120, 45.240 |
| Unit cell angles | 90.00, 106.73, 90.00 |
Refinement procedure
| Resolution | 43.310 - 1.600 |
| R-factor | 0.18918 |
| Rwork | 0.187 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x23 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.018 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.310 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | 0.123 |
| Number of reflections | 17905 | |
| <I/σ(I)> | 17.14 | 13.15 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2M sodium formate, 0.1M sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
