3L1O
Crystal structure of monoclonal antibody MN423 Fab fragment with free combining site, crystallized in the presence of zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8166 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.330, 75.426, 72.701 |
| Unit cell angles | 90.00, 92.86, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.000 |
| R-factor | 0.16237 |
| Rwork | 0.158 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v17 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.415 |
| Number of reflections | 24684 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 84.6 | 99.7 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 10% PEG 3350, 0.1M IMIDAZOLE, 0.01M ZNSO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
