3L1N
Crystal structure of Mp1p ligand binding domain 2 complexd with palmitic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97894 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.510, 46.510, 149.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.300 |
| R-factor | 0.18242 |
| Rwork | 0.182 |
| R-free | 0.19755 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.079 | 0.383 |
| Number of reflections | 40950 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 20.2 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 11% polyethylene glycol(PEG)8000, 0.1M HEPES(pH 7.5), 8% ethylene glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
