3L1L
Structure of Arg-bound Escherichia coli AdiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 84.690, 127.170, 157.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.296 - 3.002 |
| R-factor | 0.2244 |
| Rwork | 0.222 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H5M |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.646 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.296 | 3.110 |
| High resolution limit [Å] | 3.002 | 3.000 |
| Rmerge | 0.068 | 0.961 |
| Number of reflections | 17222 | |
| <I/σ(I)> | 25.8 | 2 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 0.1mM Hepes pH 7.5, 26% (w/v) PEG 600 with 0.05% DSM as additive, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
