3L19
Crystal structure of calcium binding domain of CpCDPK3, cgd5_820
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-11-18 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97948 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.570, 64.187, 72.807 |
Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
Resolution | 19.350 - 2.140 |
Rwork | 0.215 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aao |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BALBES |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.069 | 0.595 |
Number of reflections | 22779 | |
<I/σ(I)> | 17.88 | 2.99 |
Completeness [%] | 97.5 | 85.8 |
Redundancy | 7.39 | 6.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30% PEG550MME, 0.05M Magnesium Chloride, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |