3L10
Structure of split monoubiquitinated PCNA with ubiquitin in position one
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 122.516, 122.516, 122.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.000 - 2.800 |
| R-factor | 0.279 |
| Rwork | 0.277 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.587 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.630 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.109 | 0.604 |
| Number of reflections | 15333 | |
| <I/σ(I)> | 10.5 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.74 | 5.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 2.04M ammonium sulfate, 0.1M sodium citrate, 3% ethanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
