3L0U
The crystal structure of unmodified tRNAPhe from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9704 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 99.450, 99.450, 110.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.120 - 3.000 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.761 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.450 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.126 | 1.244 |
| Number of reflections | 6886 | |
| <I/σ(I)> | 12.3 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 15.7 | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.3M KCL, 0.1M Hepes pH 7.5, 35% v/v MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
