3KZW

Crystal structure of cytosol aminopeptidase from Staphylococcus aureus COL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	143.383, 154.733, 340.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.620 - 2.700
R-factor	0.22234
Rwork	0.221
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gyt
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.620
High resolution limit [Å]	2.700
Rmerge	0.139
Number of reflections	204154
<I/σ(I)>	9.19
Completeness [%]	98.8
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	70% MPD, 0.1M HEPES, pH 7.5, 0.5M sodium chloride, 0.01M Tris-HCl, pH 8.3, JCSG+, VAPOR DIFFUSION, SITTING DROP, temperature 295K