3KZW
Crystal structure of cytosol aminopeptidase from Staphylococcus aureus COL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 143.383, 154.733, 340.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.620 - 2.700 |
R-factor | 0.22234 |
Rwork | 0.221 |
R-free | 0.24020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gyt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.841 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.620 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.139 |
Number of reflections | 204154 |
<I/σ(I)> | 9.19 |
Completeness [%] | 98.8 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 70% MPD, 0.1M HEPES, pH 7.5, 0.5M sodium chloride, 0.01M Tris-HCl, pH 8.3, JCSG+, VAPOR DIFFUSION, SITTING DROP, temperature 295K |