3KZV
The crystal structure of a cytoplasmic protein with unknown function from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 117.413, 117.413, 89.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.050 - 2.000 |
| R-factor | 0.19409 |
| Rwork | 0.191 |
| R-free | 0.24461 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.284 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.050 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.148 | 0.536 |
| Number of reflections | 20048 | |
| <I/σ(I)> | 1.5 | 36.9 |
| Completeness [%] | 98.7 | 93.58 |
| Redundancy | 16.3 | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.1M Bis-tris, 5% Iso-propanol, 2.4M NH4SO4,1/1600 chymotrypsin, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
