3KZM

Crystal structure of N-acetyl-L-ornithine transcarbamylase complexed with carbamyl phosphate

Replaces:  1ZQ2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	95
Detector technology	CCD
Collection date	2004-12-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9186
Spacegroup name	I 21 3
Unit cell lengths	129.303, 129.303, 129.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.720 - 1.950
Rwork	0.197
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kzk
RMSD bond length	0.006
RMSD bond angle	1.100
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.068	0.428
Number of reflections	26292
<I/σ(I)>	29.3	4.7
Completeness [%]	99.9	100
Redundancy	9.8	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6	291	Lithium sulfate, Bis-tris, PEG3350, pH 6.0, hanging drop, temperature 291K