3KZG

Crystal structure of an arginine 3rd transport system periplasmic binding protein from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	41.965, 148.254, 149.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.310 - 2.060
R-factor	0.221
Rwork	0.221
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.310	2.130
High resolution limit [Å]	2.060	2.060
Rmerge	0.130	0.236
Number of reflections	58467
<I/σ(I)>	12.2	2.5
Completeness [%]	99.9	99.4
Redundancy	13	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	25% PEG 3350, 0.1M Bis-Tris, 0.2M Lithium sulfate monohydrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K